Gallery: Disease-Fighting Secrets of Genetically Modified Mosquitoes
01aedes-aegypti
Insects [cover our planet](http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0001495). Filed into 750,000 different species, at any given time there are one million trillion bugs buzzing around the globe. About 14,000 of those species are blood-feeders, meaning they drink their meals by puncturing the skin of vertebrates, including humans. Besides being a nuisance, blood-feeding insects, such as certain strains of mosquitoes, are infected with parasitic organisms that are no threat to the bugs themselves, but if transferred to humans, become toxic, or worse, lethal. A new [collection of studies from PLoS](http://www.ploscollections.org/GMInsect) charts alternative strategies for curbing mosquito-borne diseases such as dengue and malaria using genetically modified insects. The idea is to create mosquitoes that are less infectious and pass their altered genetics to the next generation by breeding with their natural counterparts. Even though GM insects offer potential relief from malaria and dengue, which remain untreatable, unpreventable diseases in much of the developing world, there is still a lot of skepticism, mistrust and even fear of genetically modified organisms of any sort. Careful regulation, program oversight, and public information campaigns are just as important as proving the efficacy of GM insect release. These pictures show the different types of mosquitoes whose genetic profiles are changing in the name of science and public health. __Above:__ *Aedes aegypti* --------------- *Aedes aegypti* is a carrier mosquito for the virus that causes dengue fever in humans. Researchers discovered that by introducing *Wolbachia* bacterial strains into some insects, the dengue virus didn’t live as long as usual inside the mosquitoes. What’s more, because most *Wolbachia* strains are relatively harmless to the insects, the bacteria spread through mosquito generations, passing from mother to offspring. A study in Zhiyong Xi’s lab at Michigan State University found that the *Wolbachia* bacterium [stopped the dengue virus from replicating](http://www.ploscollections.org/article/info%3Adoi%2F10.1371%2Fjournal.ppat.1000833), and therefore kept it from spreading among mosquitoes. In fact, 14 days after the study started, 37.5 percent of the mosquitoes were unable to infect humans with dengue. Another study, from Elizabeth McGraw’s lab at The University of Queensland, Australia, showed that by using a modified *Wolbachia* bacteria (wMelPop), the older mosquitoes -- the insects that typically pass dengue to humans -- [had a shortened lifespan](http://www.ploscollections.org/article/info%3Adoi%2F10.1371%2Fjournal.pntd.0000568) because they could no longer feed. From the age of 26 days onward, the mosquitoes with the wMelPop *Wolbachia* bacteria in their bodies started drinking significantly less blood than their uninfected counterparts.
02aedes-albopictus
*Aedes albopictus* ------------------ The Asian Tiger mosquito,*Aedes albopictus*, can carry diseases including dengue and chikungunya. Compared to its genetic relative *A. aegypti*, this mosquito is typically less likely to house a virus. Since *A. albopictus* carries less risk of infection, the latest advances in genetic engineering seemed to have been reserved for the more virulent *A. aegypti*. But *A. albopictus* is hardier than its relatives, and travels longer distances from the equator. In the past 30 years, researchers have watched them move throughout Southeast Asia to Northern Asia to Europe to the United States. Combined with the fact that certain mutations of the chikungunya virus are now carried by these mosquitoes, more and more researchers believe finding a suitable eradication strategy is crucial. A team of collaborators from the GM insect technology company Oxitec, Imperial College, and the University of Oxford took the first steps forward, showing they could [stably deliver a transgene to *A. albopictus* embryos](http://www.ploscollections.org/article/info%3Adoi%2F10.1371%2Fjournal.pntd.0000788), the first genetic modification ever to be done on Asian Tiger mosquitoes.
03anopheles-stephensi
*Anopheles stephensi* --------------------- *Anopheles stephensi* is a type of mosquito that [can become infected by *Plasmodium*](http://www.britannica.com/EBchecked/topic/463621/Plasmodium), parasitic protozoans that cause malaria in humans. Two studies highlight different ways to get these mosquitoes to resist infection by *Plasmodium* strains. First, George Dimopoulos’s team at Johns Hopkins University created a genetically modified mosquito by triggering a certain protein (Rel2) to [boost the bug’s immunity when it drank blood](http://www.ploscollections.org/article/info%3Adoi%2F10.1371%2Fjournal.ppat.1002458). The results showed that his genetic trigger worked: Rel2 increased after feeding, and there were fewer traces of *Plasmodium* in the genetically modified insect’s saliva compared to the controls. In a second study, led by Anthony James at UC Irvine, scientists showed that by introducing new genes that coded for immune system components from both mosquitoes and mice, they could create a transgenic [insect that was resistant to the *Plasmodium*](http://www.ploscollections.org/article/info%3Adoi%2F10.1371%2Fjournal.ppat.1002017) parasite.
04anopheles-albimanus
*Anopheles albimanus* --------------------- George Dimopoulos, the researcher who showed that tweaking the Rel2 protein in *A. stephensi* turned on the insect’s immune system after a blood meal, has also done similar work in *A. albimanus*. In his proof-of-concept studies, he discovered that artificially increasing or decreasing Rel2 caused certain of the mosquitoe's immunity genes [to turn on or off](http://www.ploscollections.org/article/info%3Adoi%2F10.1371%2Fjournal.ppat.1002458), respectively.
05anopheles-gambiae
*Anopheles gambiae* ------------------- Anopheles gambiae is the most important *Anopheles* species for malaria in Africa. A group led by Steven Sinkins at the University of Oxford found that simply introducing a modified *Walbachia* strain was enough to shorten the insect's lifespan, halt the development of the *Plasmodium* pathogen, and [activate the parts of the mosquito’s immune system](http://www.ploscollections.org/article/info%3Adoi%2F10.1371%2Fjournal.ppat.1001143) that affect whether an infective parasite will live or die within the host.
